MECHANISM-DISCOVERY EXERCISE

How does a single ORC load two helicases?

Build physical loading intermediates, trigger individual ATPase events, and compare what your model produces with structural and single-molecule observations. A successful endpoint is not enough: the path must distinguish one-ORC loading from alternative mechanisms.

WHAT IS KNOWN

Loading order seen one molecule at a time

The predominant one-ORC pathway is shown as observed arrivals and departures. The highlighted event is the next observation not yet reproduced by your model.

    Open investigation

    Assemble a candidate loading pathway

    Drag molecules into the chamber, or select one and click a position. No action is disabled.

    0/2MCM loaded
    0ORCs engaged
    0/6structures revealed
    ONE-ORC HYPOTHESIS

    No alternative-route events have been invoked.

    REACTION CHAMBER

    ARS1 origin DNA

    Begin with the origin-bound factor. ORC may form a stable contact at any horizontal position on the DNA.
    OBSERVED NOW

    Origin DNA only; no stable protein association detected.

    Build any mechanism consistent with the supplied constraints Stable associations, releases, and product geometry are recorded without marking each move right or wrong.

    MECHANISM QUESTIONS

    Explain what the loading pathway reveals

    Use your model and the structures you revealed. Each response is evaluated independently by an automated feedback service.

    Submitted answer text is processed only to generate feedback. Do not include personal or sensitive information.

    ALTERNATIVE MODEL

    You have invoked a second ORC

    MODEL EVALUATION

    What does this run establish?

    DEPOSITED 3D RECONSTRUCTION

    Cryo-EM structure